• Reconstitute your 500 mg NAD⁺ vial with 3 mL of bacteriostatic water (BAC) as the standard dilution.
  Gently swirl (do not shake) until fully dissolved.
• Since NAD⁺ may cause a burning or stinging sensation, you can add up to 6 mL of BAC water to further dilute the solution and make injections more comfortable.
• Store it in the refrigerator (2–8 °C). DO NOT FREEZE.
• Use only within 28-30 days upon mixing.

Best Time to Inject

• Morning or early afternoon is ideal as NAD⁺ can boost energy and alertness.
  
• Avoid injecting right before bedtime, as it may cause mild insomnia or restlessness.
  

Injection Site

Subcutaneous (subq) only — inject into fatty areas such as:

• Lower abdomen
• Outer thigh
• Back of upper arm

How to Inject

1. Pinch a small fold of skin between your thumb and index finger.
   
2. Insert the needle at a 45–90° angle into the subcutaneous tissue.
   
3. Inject slowly to reduce the burning or tingling sensation.
   
4. Withdraw the needle gently and apply light pressure with a clean cotton or alcohol swab.

Rotate sites with each dose to prevent irritation.

• Inject slowly: NAD⁺ can cause a mild burning or tingling sensation if injected too fast. Take your time! slow, steady pressure helps minimize discomfort.
• Stay hydrated: Drink at least 1–2 liters of water daily. NAD⁺ increases cellular metabolism, so hydration supports detoxification and energy balance.
• Start low, go slow: Begin with a low dose and increase gradually based on your tolerance. A 100 mg dose isn’t required for everyone.
• Expect temporary fatigue: You might feel slightly lethargic as your body adjusts to higher NAD⁺ levels — this usually improves as your cells adapt.
• Rotate injection sites: Alternate between the abdomen, thighs, or upper arms to avoid irritation or bruising.
• Ensure sterility: Use new alcohol wipes, sterile needles, and syringes every time. Discard sharps safely after use.
• Best time to inject: Morning or early afternoon is ideal. Avoid injecting right before bedtime to prevent potential sleep disturbance.
• Storage: Keep your reconstituted vial refrigerated (2–8 °C). Use within 28 days after mixing with bacteriostatic water.
• Optional synergy tip: For those stacking peptides, inject NAD⁺ first before compounds like Tesamorelin or Ipamorelin to prime mitochondrial energy.

• Stay well-hydrated — aim for 1–2 L of water daily.
  
• Mild warmth, tingling (even on the tongue), or fatigue can occur — this is normal and temporary.
  
• Do not exceed your tolerance. Adjust your dose or dilution (up to 6 mL BAC water) if injections feel uncomfortable.
  
• Store the vial in the refrigerator (2–8 °C). Use within 20 days after reconstitution.
  

• A warm or tingling feeling under the skin or on the tongue can occur as NAD⁺ begins circulating — this is normal and temporary.
  
• It’s a sign of increased cellular activity and blood flow.
  
• The sensation typically fades within a few minutes.
  
• If it becomes uncomfortable, slow your injection rate and stay hydrated before your next dose.
  

The timeframe to notice effects from NAD+ supplementation can vary a lot depending on the dose, route (IV vs subcutaneous vs oral), your baseline NAD levels, and overall health. Here’s a detailed breakdown:

1. Immediate / Short-Term Effects (Within Hours to Days)

• Mental clarity, alertness, focus – some people notice subtle cognitive improvements after their first IV infusion or injection.
  
• Mild energy boost – feeling slightly less fatigued, more awake.
  
• Possible mild side effects – flushing, tingling, nausea, or headache as your body adjusts.
  

2. Short-Term (1–2 Weeks)

• Improved sleep quality for some users.
  
• Better mood stability or slight reduction in anxiety/stress.
  
• Reduced brain fog or improved memory recall.

3. Medium-Term (3–6 Weeks)

• Increased physical energy and endurance.
  
• Enhanced metabolism and possibly better exercise recovery.
  
• Skin improvements (less dullness, more hydration) may start to appear for some.
  

4. Long-Term (2–3 Months or more)

• Stronger cellular resilience and repair (mitochondrial function).
  
• Noticeable anti-aging and cognitive benefits.
• Potential improvements in chronic fatigue, metabolic health, and inflammatory markers.

Benefits are gradual and cumulative; don’t expect dramatic effects in the first few days. Effects may vary individually depending on age, baseline NAD+ levels, and overall health.